Figure 4
NF-κB signals are required for LMP1-mediated Fascin induction. (A) Quantitative PCR of Fascin mRNA in Jurkat cells after transfection of wt-LMP1 (pSV40-LMP1) and co-transfection of pIκBα-DN or treatment with the IKKβ inhibitor ACHP (2-Amino-6-(2-(cyclopropylmethoxy)-6-hydroxyphenyl)-4-(4-piperidinyl)-3-pyridine-carboni-trile) solved in DMSO. ACHP (10 μM) was added 24 h after transfection for 24 h. Relative copy numbers were determined by normalizing Fascin transcripts to those of ACTB. Mean values +/− SE were compared using a t-test (n = 4). **indicates P < 0.01. (B) Immunoblot of Fascin, LMP1, p100, p52, and ACTB (ß-Actin) in Jurkat cells after transfections as described in (A). LMP1 expression was quantitatively evaluated by densitometry and normalized on ACTB protein expression. The mean of four independent experiments +/−SE is shown and was compared (t-test, n = 4, P > 0.05). n.s. indicates not significant.