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Figure 7 | Cell Communication and Signaling

Figure 7

From: Integration of proteomic and transcriptomic profiles identifies a novel PDGF-MYC network in human smooth muscle cells

Figure 7

DIAPH3 is a novel target of PDGF that regulates lamellipodium formation. (A) Predictive model for MYC-JUN-DIAPH3 pathway in response to PDGF in pBSMC. (B) Immunoblot analysis depicting kinetics of DIAPH3 expression in pBSMC pre-treated with vehicle, JNK inhibitor (JNKi) or MYC inhibitor (MYCi) and subsequently treated with PDGF for 4 h or 24 h (C, D) Representative immunofluorescence images of pBSMC with (C, arrow) or without (D) lamellipodium formation. (E) Silencing of DIAPH3 attenuates the PDGF-mediated increase in lamellipodia formation in pBSMC (lower panel). Upper panel: immunoblot confirming efficiency of DIAPH3 knockdown in pBSMC. **, p < 0.05.

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Cell Communication and Signaling

ISSN: 1478-811X