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Table 1 Modified working volumes for Click IT cocktail for EdU analysis of S phase.

From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

Reaction components for Click IT cocktail   Number of reactions  
  2 5 10
10× Click-IT Reaction Buffer 87.5 ul 219 ul 438 ul
CuSO4 20 ul 50 ul 100 ul
Fluorescent dye 5 ul 12.5 ul 25 ul
10× Reaction buffer additive 10 ul 25 ul 50 ul
Water 877.5 ul 2193.5 ul 4387 ul
TOTAL volume 1 ml 2.5 ml 5 ml