Table 1 Modified working volumes for Click IT cocktail for EdU analysis of S phase.
From: A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells
Reaction components for Click IT cocktail | Â | Number of reactions | Â |
|---|---|---|---|
| Â | 2 | 5 | 10 |
10× Click-IT Reaction Buffer | 87.5 ul | 219 ul | 438 ul |
CuSO4 | 20 ul | 50 ul | 100 ul |
Fluorescent dye | 5 ul | 12.5 ul | 25 ul |
10× Reaction buffer additive | 10 ul | 25 ul | 50 ul |
Water | 877.5 ul | 2193.5 ul | 4387 ul |
TOTAL volume | 1 ml | 2.5 ml | 5 ml |