Molecular mechanisms of gastric epithelial cell adhesion and injection of CagA by Helicobacter pylori

Table 2 Reported phosphorylation/injection of CagA in human cell lines^a

Cell line	Origin	H. pylori strains used	Phospho-CagA^b	Applied methods	References
AGS	stomach	G27, P1, P12, P210, GU301, GU303, GU304, GU305, GU306, GC401, GC402, 342, ATCC43579, 87A300, NCTC11916, NCTC11637	+++	IP, Anti-PY WB, CF, IF, 2-DE, MS	[78, 92–95]
MKN-1	stomach	P12	+	IP, Anti-PY WB	[75]
MKN-7	stomach	P12	+	IP, Anti-PY WB	[75]
MKN-28	stomach	P1, P12	++	Anti-PY WB/IPA	[74]
MKN-45	stomach	P1, P12	+++	Anti-PY WB/IPA	[74]
MKN-74	stomach	P12	+	IP, Anti-PY WB	[75]
KATO-3	stomach	P1, P12	+++	Anti-PY WB/IPA	[74]
23123/87	stomach	P12	++	IP, Anti-PY WB	[75]
Hs-746T	stomach	P12	++	IP, Anti-PY WB	[75]
FU-97	stomach	P12	+	IP, Anti-PY WB	[75]
NUGC-4	stomach	P12	+	IP, Anti-PY WB	[75]
SNU-1	stomach	P12	+	IP, Anti-PY WB	[75]
SNU-5	stomach	P12	++	IP, Anti-PY WB	[75]
SNU-16	stomach	P12	+	IP, Anti-PY WB	[75]
HeLa	cervix	P1, P12	++	Anti-PY WB/IPA	[74, 84]
HT-29	colon	P1, P12	++	Anti-PY WB/IPA	[74]
Hec1.b	endometrium	P1, P12	+	Anti-PY WB/IPA	[74]
293T	kidney	P1, P12	++	Anti-PY WB/IPA	[74]
HL	lung	P1, P12	++	Anti-PY WB/IPA	[74]
HepG2	liver	P1, P12	++	Anti-PY WB/IPA	[74]
MCF-7	breast	P12	+++	Anti-PY WB	[72]
THP1	blood	P1, P12, ATCC43526	+++	Anti-PY WB	[96]
U937	blood	P1, P12, G27	+++	Anti-PY WB	[97]
Josk-M	blood	P1, P12, G27	+++	Anti-PY WB	[97]
B cells, primary	blood	HM-6, HM-9	+++	IP, Anti-PY WB	[98]
BJAB	blood	HM-6, HM-9	+++	IP, Anti-PY WB	[98]

^a Abbreviations: 2-DE (two-dimensional protein gel electrophoresis); anti-PY WB (Westernblotting of infected cell lysates using several pan-phospho-tyrosine antibodies); CF (cell fractionation); IF (localization by immunofluorescence); IP (immunoprecipitation); IPA (in vitro phosphorylation assays of CagA with cell lysates to ensure that active kinases are present in the cells of interest); MS (mass spectrometry of precipitated phospho-CagA).
^b Intensity of CagA's tyrosine phosphorylation signals: +++ strong phosphorylation; ++ moderate phosphorylation; + weak phosphorylation.

ISSN: 1478-811X