Figure 8
From: LiCl induces TNF-α and FasL production, thereby stimulating apoptosis in cancer cells
LiCl increases the amount of Survivin and of phosphorylated ERK. (A) HCT116 wild type and HCT116 p53-/- cells were incubated with 50 mM LiCl for the indicated times. Cell lysates were prepared and 50 μg of protein were separated on a 10% SDS-PAGE gel. Proteins were transferred onto a PVDF membrane and probed with antibodies directed against Survivin and Bcl-XL, and against PCNA for a loading control. (B) HCT116 wild type and HCT116 p53-/- cells were incubated with the indicated concentrations of LiCl for 48 hours. Cell lysates were prepared and 50 μg of protein were separated on a 10% SDS-PAGE gel. Proteins were transferred onto a PVDF membrane and probed with antibodies directed against Survivin and Bcl-XL, and against PCNA for a loading control. (C) U2OS cells were treated with 50 mM LiCl and harvested at the indicated time points. Cell extracts were prepared and 50 μg of protein were separated on a 10% SDS-PAGE gel. Proteins were transferred onto a PVDF membrane and probed with an antibody directed against phosphorylated ERK. The membrane was stripped and reprobed with an antibody directed against the p42 and p44 kDa forms of ERK, for a loading control.