Figure 3
From: Src mediates cytokine-stimulated gene expression in airway myocytes through ERK MAPK
Effects of PP1 on cytokine-stimulated MAPK phosphorylation. Human airway myocytes were treated for 15 minutes with 10 ng/ml IL-1β, TNFα and IFNγ in the presence of the 0.1% DMSO vehicle + 30 μM PP1. Aliquots of whole cell lysates were separated by SDS-PAGE and immunoblots performed to examine phosphorylation of ERK (5 μg total protein) and p38 MAPKs (15 μg total protein). A. Effect of PP1 on cytokine-stimulated ERK 1/2 MAPK phosphorylation (pERK 1/2) normalized to non-phosphorylated ERK. B. Effect of PP1 on cytokine-stimulated p38 MAPK phosphorylation (p-p38) normalized to non-phosphorylated p38 MAPK. Representative immunoblots are shown along with normalized densitometric data expressed relative to the immunoreactivity seen in DMSO treated control cultures, n = 4 + SEM. * indicates significant difference from cytokine-stimulated group, P < 0.05.