Figure 2

Specific regulation of cofilin, Cdc2, and stathmin phosphorylation in scrapie-infected neuronal cells. Cell lysates of N2a58# and 22L-infected N2a58/22L cells were analyzed by Western blot using phospho-specific antibodies to detect p-cofilin^S3, p-cdc2^Y15, and p-stathmin^S38 (left panels). As loading controls, equal amounts of cofilin, Cdc2 and stathmin were shown. Quantification of intensities of phosphorylation signal was performed by normalizing the corresponding loading control (* p < 0.05) (right panels).