Volume 7 Supplement 1

12th Joint Meeting of the Signal Transduction Society (STS). Signal Transduction: Receptors, Mediators and Genes

Open Access

Fungal GPCR signaling in pheromone response

  • E Kothe1,
  • S Erdmann1,
  • N Knabe1 and
  • E-M Jung1
Cell Communication and Signaling20097(Suppl 1):A62

https://doi.org/10.1186/1478-811X-7-S1-A62

Published: 26 February 2009

The mushroom Schizophyllum commune shows several phenotypes linked to pheromone signaling and Ras-dependent development. The genes involved in MAPK signaling, cAMP dependent signaling and Rho signaling all turned on after pheromone recognition in a Ras-dependent manner have been analyzed using the genome sequence. Receptor-pheromone recognition leads to mating and is dependent in mated dikaryons for nuclear migration and clamp cell fusion. In vitro mutagenesis could show domains in the protein involved in ligand recognition. Receptor localization and expression studies could elucidate the involvment in clamp cell fusion. The signal is transmitted through a MAPK cascade and via Ras. Ras constitutive mutant alleles transformed into a wildtype recipient as well as deletion of a RasGap lead to enhanced intracellular signaling. By this approach phenotypes in clamp cel fusion, mushroom development and meiosis, as well as hyphal directional growth can be linked to Ras signaling.

The genome sequence allowed us to identify components, which are used for expression studies during pheromone response and mating. A proteome analysis additionally is used to define components involved in pheromone response in this easily tractable fungus.

Authors’ Affiliations

(1)
Friedrich-Schiller-University, Microbiology

Copyright

© Kothe et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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