Volume 7 Supplement 1

12th Joint Meeting of the Signal Transduction Society (STS). Signal Transduction: Receptors, Mediators and Genes

Open Access

The role of STAT6 in human T cell transformation by herpesvirus saimiri

  • ED Mazumder1,
  • B Scholz1,
  • E Heck1,
  • D Lengenfelder1,
  • M Schmidt1,
  • I Müller-Fleckenstein1,
  • B Fleckenstein1,
  • B Biesinger1 and
  • A Ensser1
Cell Communication and Signaling20097(Suppl 1):A51

https://doi.org/10.1186/1478-811X-7-S1-A51

Published: 26 February 2009

Herpesvirus saimiri (HVS) induces T-cell lymphoma in New World primates. HVS subgroup C strains growth transform human T-cells in vitro. Oncoproteins StpC and Tip are essential for transformation. The lymphocyte tyrosine kinase Lck is the major interaction partner of Tip and phosphorylates Tip at specific tyrosine residues. We find that STAT6 is activated by Tip together with Lck, requiring either Tip residues, Y114 or Y127 for increased activation and both for full activation.

Our analysis addresses whether Tip or the Y114F and/or Y127F mutant mediate interactions between STAT6 and Lck or other Src family members. The components are expressed and purified using the Strep-tag system to identify additional factors involved in complex formation by affinity chromatography. Furthermore, respective fluorescent fusion proteins are made to observe colocalization patterns and interaction within living T cells by fluorescence resonance energy transfer. These studies on the association of Tip, Lck, and STATs can resolve further regulatory mechanisms involved in viral transformation process.

Authors’ Affiliations

(1)
Universitätsklinikum, Virologisches Institut

Copyright

© Mazumder et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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