- Meeting abstract
- Open Access
Human calreticulin can act as adjuvant in the maturation of antigen presenting cells
© Bajor et al; licensee BioMed Central Ltd. 2009
- Published: 26 February 2009
- Endoplasmic Reticulum
- Cell Culture Supernatant
- Scale Expression
- Protein Retention
- shRNA Sequence
Calreticulin (CRT), an endoplasmic reticulum (ER) resident protein, is involved in critical cellular functions, such as protein folding and antigenic peptide cross presentation. Furthermore, this chaperone has been proposed to act as an adjuvant during the activation of dendritic cells (DCs) in vivo. We assessed human eukaryotically expressed CRT for its potential to induce NF-kappa B regulated maturation of monocyte-derived DCs. In order to facilitate eukaryotic expression procedures, we established and compared three different methods to express recombinant endotoxin-free CRT to be secreted in the supernatant of HEK 293 cells: (1.) the complete, unmodified CRT coding sequence was cloned into the pcDNA3.1V5/His vector (euCRT), (2.) the C-terminal ER-retrieval KDEL amino acid sequence was mutated into KDQL in order to disturb the endoplasmic retention and support the protein secretion (euCRT_KDQL) and (3.) a shRNA was designed to knock down the expression of aminoacyl-tRNA synthetase-interacting multifunctional protein-1 (AIMP-1), which is known to regulate protein retention in the ER. An efficient shRNA sequence specific to AIMP-1 transcripts was delivered to HEK 293 cells, which were afterwards transfected with the CRT-expressing vector (euCRT). No relevant differences between these different approaches were observed in regard to mRNA levels of the transfected CRT determined by Real Time RT-PCR as well as protein expression levels of CRT determined by V5/HIS ELISA in the cell culture supernatants. Thus, for large scale expression of CRT the first strategy with the unmodified CRT sequence (euCRT) was chosen. The functional capability of the expressed calreticulin to induce maturation of DCs was tested. By flow cytometry the translocation of NF-kappa B into the nuclei of the monocytes after stimulation with the recombinant CRT could be demonstrated. Using low-dose CRT (10 μg/ml) the phenoptype of the immature DCs changed to a more matured one, as indicated by an increased surface expression of CD40, CD86, CD83. In summary, our first data indicate, that this recombinant CRT can act as an adjuvant for in vitro maturation of DCs and therefore has the potential to assist in T cell stimulation and expansion protocols.
This article is published under license to BioMed Central Ltd.