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Figure 4 | Cell Communication and Signaling

Figure 4

From: Rac1 activation inhibits E-cadherin-mediated adherens junctions via binding to IQGAP1 in pancreatic carcinoma cells

Figure 4

Immunofluorescence localisation of E-cadherin, β-catenin, IQGAP1 and filamentous actin (F-actin). (A) PANC-1 cells stably expressing EGFP, EGFP-Rac1(V12) or EGFP-Rac1(N17) were incubated with specific antibodies against E-cadherin, β-catenin, IQGAP1 or CPTIC-conjugated phalloidin. The staining was examined by fluorescence microscopy (bar = 20 μm). (B) EGFP-Rac1(V12)-expressing PANC-1 cells were stained for IQGAP1 and F-actin with CPTIC-conjugated phalloidin. The merged image shows in yellow the colocalisation of IQGAP1 and filamentous actin (bar = 10 μm).

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Cell Communication and Signaling

ISSN: 1478-811X