Figure 1

αvβ3 integrin-dependent invasion of C8161.9 cells. A) Cell surface expression levels of αvβ3 (clear bar) and αvβ5 (black bar) integrins in two metastatic melanoma cell lines, C8161.9 and M14-mel, normal cultured melanocytes (NHEM), and the endothelial cell line HUVEC, were measured using FACS after surface labeling with fluorescently-labeled anti-integrin antibodies. B) Vitronectin-induced Matrigel invasion of C8161.9, M14, or normal cultured melanocytes (NHEM) in Boyden chambers in the absence of serum or growth factors was assessed in the absence (IgG) or presence of anti-integrin blocking antibodies (anti-αvβ3 or anti-αvβ5). In the absence of vitronectin (no VN), cells were unable to invade the Matrigel.