Figure 5

Inhibition of Erk activation suppresses cell death induced by combining cisplatin and GSI. Cells were treated as in Figure 4, or additionally pre-incubated for 1 h with the Mek inhibitor UO126 (30 μM) where indicated and digital images recorded (10× objective). Cells were harvested after 24 h and equal amounts of RIPA lysates (50 μg) analysed for pErk and cleaved PARP as before. UO126 blocks the induction of pErk by DBZ and cisplatin, reduces the level of cleaved PARP and suppresses the cell death induced by the combination of these drugs.