Figure 4

Combination of Cisplatin with DBZ, a potent GSI, elicits a striking induction of cell death. Cells were treated as indicated and digital images recorded (20× objective). 10 μM Cisplatin (CP) alone leads to a moderate degree of cell death. Note that less cells are present in cultures treated with cisplatin (upper left panel) compared to untreated or DBZ treated cultures (upper right panel). The cells appear flatter and the nuclei larger. A section of the plate was chosen where only few dead cells obstruct the view onto the remaining cells (see Figure 5 for a lower magnification view of CP treated cells). The combination of cisplatin with DBZ leads to massive cell death.