Figure 5

Treatment of SW620 cells with the SFK inhibitor PP2 abolishes the tyrosine phosphorylation of Odin. SW620 cells were treated with 30 μM of the SFK inhibitor PP2 dissolved in DMSO or 0.06%(v/v) DMSO for 24 h, then lysed and subjected to immunoprecipitation with anti-Odin or normal rabbit IgG (contr. IgG) as indicated. pY protein was detected by western blotting with anti-pY mAb. 10 μg of untreated SW620 TCL were also analysed. The blot was subsequently stripped and reprobed to detect Odin. The black arrowheads indicate the Odin bands, the open arrowhead points to a protein that binds non-specifically to control IgG beads and is equally affected by PP2 treatment of the SW620 cells.