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Figure 3 | Cell Communication and Signaling

Figure 3

From: Correction: RNA interference-mediated gene silencing in murine T cells: in vitro and in vivo validation of proinflammatory target genes

Figure 3

RNAi is applicable for signalling proteins involved in T cell activation. Stimulated murine T cells were transfected with 0.9 μM ZAP70-, PLC-γ- or Lck-specific siRNA pool by using nucleofection. Cell lysates were prepared 24 and 48 h after transfection and mRNA and protein expression were analyzed by RT-PCR (A) and Western blot analysis (B). As control, cells were transfected with a control siRNA pool (Ctrl). (A) RT-PCR results of transfected T cells determined 24 h after nucleofection with Control ("siCtrl"), ZAP70 ("siZAP70"), PLC-γ ("siPLC-γ1") and Lck ("siLck") specific siRNAs. (B) Western blot analysis of transfected T cells 48 h after nucleofection. Protein knockdown of ZAP70, PLC-γ and Lck was detected by specific antibodies. β-actin was used as loading control. Shown are the mean values of triplicates of a representative experiments of two independent experiments.

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Cell Communication and Signaling

ISSN: 1478-811X