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Figure 1 | Cell Communication and Signaling

Figure 1

From: An active form of Vav1 induces migration of mammary epithelial cells by stimulating secretion of an epidermal growth factor receptor ligand

Figure 1

Vav1Y3F stimulates morphological changes and increases migration of MCF-10A cells. (A) Schematic illustration of mutations made in different domains of Vav1. The domain structure of Vav1 is illustrated at the top and the positions of residues mutated are indicated by asterisks. (B) The morphology of MCF-10A cells expressing GFP is shown in the left panels and of cells expressing Vav1Y3F is shown in the right panels. Note that cells expressing Vav1Y3F are flatter and more spread and have more ruffles and lamellipodia than the cells expressing GFP. Data are representative of more than 10 independent experiments. (C) MCF-10A cells expressing GFP and Vav1Y3F were seeded in transwell chambers and allowed to migrate overnight towards assay media without or with 20 ng/ml EGF. The average number of cells migrated in five 20x fields per transwell was determined. Migration is expressed as the percentage of migration of Vav1Y3F cells in media without EGF (set to 100%) for each individual experiment. Data are the average plus standard deviation of 8 independent experiments.

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Cell Communication and Signaling

ISSN: 1478-811X