Figure 5
From: The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxide
The effect of estradiol on eNOS mRNA and protein expression. BeWob30 cells were maintained in culture and exposed to estradiol (10 nM) as described. Cellular protein and mRNA were obtained and assessed for eNOS protein by immunoblot analysis and mRNA transcripts by semi-quantitative RT-PCR. Each lane represents a unique sample and is representative of 2 separate experiments. C1, 2, 3 = individual control samples; E1,2,3 = individual E2-treated samples