Table 2 Primers used for RT-PCR of markers for MSC-derived differentiation pathways
Alkaline phosphatase | Â | Â |
sense | 5'TGGAGCTTCAGAAGCTCAACACCA 3' | 368 – 391 NM_000478 |
antisense | 5'ATCTCGTTGTCTGAGTACCAGTCC 3' | 798 – 821 NM_000478 |
Osteocalcin | Â | Â |
sense | 5'ATGAGAGCCCTCACACTCCTC 3' | 19 – 39 NM_199173 |
antisense | 5' GCCGTAGAAGCGCCGATAGGC 3' | 292 – 312 NM_199173 |
Lipoprotein lipase | Â | Â |
sense | 5'GAGATTTCTCTGTATGGCACC 3' | 1261 – 1281 NM_000237 |
antisense | 5'CTGCAAATGAGACACTTTCTC 3' | 1516 – 1536 NM_000237 |
PPARγ2 |  |  |
sense | 5'GCTGTTATGGGTGAAACTCTG 3' | 128 – 148 NM_015869 |
antisense | 5'ATAAGGTGGAGATGCAGGCTC 3' | 458 – 478 NM_015869 |
Collagen type II | Â | Â |
sense | 5'GAACATCACCTACCACTGCAAG 3' | 4318 – 4339 NM_001844 |
antisense | 5'GCAGAGTCCTAGAGTGACTGAG 3' | 4684 – 4705 NM_001844 |
Collagen type X | Â | Â |
sense | 5'CCCTTTTTGCTGCTAGTATCC 3' | 112 – 132 NM_000493 |
antisense | 5'CTGTTGTCCAGGTTTTCCTGGCAC 3' | 556 – 579 NM_000493 |
Collagen type XI | Â | Â |
sense | 5'ACTTCTGACTGCCTCTGCTC 3' | 5418 – 5437 NM_001854 |
antisense | 5'GCTTTTGCCATGTGATTCTGCC 3' | 5891 – 5912 NM_001854 |
Aggrecan | Â | Â |
sense | 5'GCCTTGAGCAGTTCACCTTC 3' | 1814 – 1833 NM_001135 |
antisense | 5'CTCTTCTACGGGGACAGCAG 3' | 2186 – 2205 NM_001135 |
Chondroadherin | Â | Â |
sense | 5'ACCTGGACCACAACAAGGTC 3' | 535 – 554 NM_001267 |
antisense | 5'CACCTTCTCCAGGTTGGTGT 3' | 907 – 923 NM_001267 |
Fibromodulin | Â | Â |
sense | 5'CTTACCCCTATGGGGTGGAT 3' | 175 – 194 NM_002023 |
antisense | 5'AAGTAGCTATCGGGGACGGT 3' | 792 – 811 NM_002023 |