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Table 1 PCR primers used for the detection of IL-8 and of T-type Ca++-channel α1 subunits.

From: Intracellular calcium changes induced by the endozepine triakontatetraneuropeptide in human polymorphonuclear leukocytes: role of protein kinase C and effect of calcium channel blockers

  Primer sequence Annealing temperature PCR product Ref.
IL-8 5'-CCACCCATGGCAAATCCATGGC-3'
5'-TCTCAGCCCTCTTCAAAAACTTCTC-3'
65°C 289 [31]
α1G 5'-CCTGGACTTCTTCACGATGT-3'
5'-CCAGGTCTGCTGGGTCAGAG-3'
63°C 395 [32]
α1H 5'-TGTTCGTGACGGACTCGAATT-3'
5'-AGTGCACAGAGGCAACGGA-3'
63°C 436 [32]
α1I 5'-TTCCCCTACACCGGAACGG-3'
5'-TAGTAACGGTTCCAGTTGA-3'
50°C 227 [32]
HPRT 5'-CCTGCTGGATTACATTAAAGCACTG-3'
5'-CTTCGTGGGGTCCTTTTCACCAGC-3'
65°C 370 Genbank n. M26434
β-actin 5'-GGAAATAGGGGTTAGCAC-3'
5'-CTCATGTGCGCCTACTTA-3'
56°C 929 Genbank n. AJ005353