Skip to main content

Table 1 PCR primers used for the detection of IL-8 and of T-type Ca++-channel α1 subunits.

From: Intracellular calcium changes induced by the endozepine triakontatetraneuropeptide in human polymorphonuclear leukocytes: role of protein kinase C and effect of calcium channel blockers

 

Primer sequence

Annealing temperature

PCR product

Ref.

IL-8

5'-CCACCCATGGCAAATCCATGGC-3'

5'-TCTCAGCCCTCTTCAAAAACTTCTC-3'

65°C

289

[31]

α1G

5'-CCTGGACTTCTTCACGATGT-3'

5'-CCAGGTCTGCTGGGTCAGAG-3'

63°C

395

[32]

α1H

5'-TGTTCGTGACGGACTCGAATT-3'

5'-AGTGCACAGAGGCAACGGA-3'

63°C

436

[32]

α1I

5'-TTCCCCTACACCGGAACGG-3'

5'-TAGTAACGGTTCCAGTTGA-3'

50°C

227

[32]

HPRT

5'-CCTGCTGGATTACATTAAAGCACTG-3'

5'-CTTCGTGGGGTCCTTTTCACCAGC-3'

65°C

370

Genbank n. M26434

β-actin

5'-GGAAATAGGGGTTAGCAC-3'

5'-CTCATGTGCGCCTACTTA-3'

56°C

929

Genbank n. AJ005353