Figure 9
From: G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane
The N-terminus of ErGPCR is the key domain for its cell membrane localization and function. (A) mutation sites of ErGPCR, △20-197 indicates the truncation of amino acids 20–197; △275-308 indicates the truncation of amino acids 275–308. (B) The localization of the overexpressed GFP (OVGFP), full length of ErGPCR fused with GFP (OVErGPCR-GFP), truncation of N-terminal extracellular region of ErGPCR fused with GFP (OVErGPCR△20-197aa-GFP), and truncation of the second inner loop of ErGPCR fused with GFP (OVErGPCR△276-308aa-GFP) in the HaEpi cells by confocal microscope. (C) Subcellular localization of ErGPCR and two mutants were confirmed by western blot with His-tag antibody. (D) The effects of the mutations of ErGPCR on the transcripts of EcRB1, BrZ2, HHR3 and USP1 induced by 20E were examined by qRT-PCR, with β-actin as control. Bars indicate mean ± S.D. from three independent experiments. Asterisk indicates significant difference (p < 0.05).