Figure 3
From: G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane
Hormonal induction of ErGPCR in the midgut by injection of 20E (A) or JH III (B) or 20E + JH III (C) into the 6th instar 6 h larvae (500 ng/larva) analyzed by qRT-PCR. DMSO is a solvent control. β-actin gene was used as the quantitative control for the mRNA. The asterisks indicate significant differences between 20E treatment and DMSO solvent control by student’s t test analysis from three independent repeats (*p < 0.05, n = 3).