Figure 10

Binding assay of [³H]Pon A to whole cells or membrane fractions. (A) Western blot analysis of the overexpressed GFP, ErGPCR-GFP and EcRB1-GFP. (B) Binding of [³H]Pon A to the whole HaEpi cells that expressed GFP, ErGPCR-GFP or EcRB1-GFP, respectively. The cells (1–100 × 10⁴) were incubated with 0.1 nM [³H]Pon A (5740 cpm) for 1 h at 27°C, respectively. (C) The membrane fractions (5, 50, 250, 500 μg membrane proteins) isolated from above cells were incubated with 0.1 nM [³H]Pon A for 1 h at 27°C, respectively. cpm: counts per minute of [³H]Pon A. The data marked with different small letters indicate a significant difference at 0.05 levels by the one-way ANOVA with Duncan′s test.