Figure 1
From: G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane
Involvement of GPCRs in the 20E pathway in HaEpi cells as determined by quantitative real-time reverse transcription polymerase chain reaction ( qRT-PCR) analysis. DMSO treatment was used as the solvent control for 20E. DMSO plus suramin 50 μM treatment for 1 h was used to determine the toxic effects of suramin on the cells. The HaEpi cells were pretreated with 50 μM suramin for 1 h and then exposed to 1 μM 20E for another 6 h. The results are based on the ΔΔCT calculation by normalization of the β-actin gene. Error bars represent the standard deviation of three independent replicates. Asterisks indicate significant differences (Student’s t test, *p < 0.05).