Figure 7
From: The Dictyostelium discoideum RACK1 orthologue has roles in growth and development
D. discoideum cells overexpressing DdRACK1 show delays and alterations in formation of developmental patterns. (A) 5 × 107 AX2, AX2/GFP, AX2/GFP-DdRACK1 and AX2/GFP-DdRACK1mut cells were deposited on phosphate agar plates and imaged at the indicated hours of development. Scale bar, 250 μm. (B) 1 × 107D. discoideum strains were starved on petri dishes under phosphate buffer. Images were taken at the indicated hours. Scale bar, 250 μm. (C) Time-dependent expression of csA. Cells from D. discoideum were collected during development in shaking suspension at the indicated time points and analyzed by SDS-PAGE and western blot. csA was detected by mAb33-294, mAb 188-19-95 detected cap32 which was used as loading control. Since cap32 blot was similar for all strains, only the cap32 blot for AX2 is shown. (D) Cell samples of D. discoideum strains were collected from submerged cultures in phosphate buffer and tested for csA expression. The cap32 blot for AX2 is shown.