Figure 6
From: The Dictyostelium discoideum RACK1 orthologue has roles in growth and development
D. discoideum cells overexpressing DdRACK1 have growth and developmental defects. (A) Levels of DdRACK1 overexpression. Cell lysates from vegetative AX2, AX2/GFP-DdRACK1 and AX2/GFP-DdRACK1mut strains were analyzed by SDS-PAGE and western blot. DdRACK1 and GFP-fusion proteins at 36 and 66 kDa were detected with polyclonal anti-DdRACK1 antibodies. (B) Growth in shaking suspension of D. discoideum strains. 5 x 104cells/ml were used for inoculation. (C) Growth of D. discoideum strains on lawns of K. aerogenes. Images were taken on days 4 and 5. Scale bar, 1 mm. (D) Bar chart showing diameter of plaques formed by D. discoideum strains in (C) measured between days 4 and 7. The bar represents the mean and SD of ten independent experiments (NS, not significant; P > 0.05). (E) Growth of D. discoideum strains on lawns of E. coli B12 and imaged on days 3 and 6. Scale bar, 1 mm. (F) Bar chart showing diameter of plaques formed by D. discoideum strains in (E) measured between days 4 and 9. The bar represents the mean and SD of ten independent experiments (**P < 0.01; *P < 0.05; NS, not significant; P > 0.05).