Figure 1

Characterization of ASC-derived EVs subpopulations, collected after 10k and 100k g ultracentrifugation. A: NanoSight representative images of 10k, 100k, and total 100k fractions of b-EVs and PDGF-EVs; B: diagram of EV quantity per single ASC after stimulation with different growth factors. Calculations of EVs were performed after ultracentrifugation of conditioned media. In selected experiments ASCs were pretreated with blocking antibodies anti PDGFRα/β and then stimulated with PDGF (+ab + PDGF), (mean ± SEM, * - p < 0,05, n = 12); C: diagram of concentration of EV 10k, 100k g and 100k total fractions in conditioned media of ASC (mean ± SEM, * - p < 0,05, n = 10); D: representative western blot analysis of exosome marker expression in the two fractions of EVs and in ASCs (10 μg of proteins/well; image of ponseau red staining in Additional file 1: Figure S1A); E: diagram of length of vessel-like structures after HMEC stimulation with different EV fractions; as control non-stimulated HMEC were used (mean ± SEM, * - p < 0,05, n = 5).