Figure 4

The induction of MAT promotes tumor growth. A) Analysis of EphA2 expression by immunoblot analysis of cells serum starved for 48 h with or without 50 μmol/L Ilomastat, or treated with 1 U/ml Calpeptin for 2 h at 37°C. An anti-actin antibody was used to ensure equal protein loading. B) Tumour incidence in SCID bg/bg mice injected with 10⁴ and 10³ EphA2 overexpressing cells or control cells. C-D) Xenograft growth in SCID bg/bg mice of EphA2 overexpressing cells or control cells s.c. injected with Matrigel in the flanks of mice. The onset and the primary tumour growth are reported. Student t-test, *p ≤ 0.001 EphA2 overexpressing cells vs control cells, #p < 0.005 EphA2 overexpressing cells vs control cells.