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Figure 5 | Cell Communication and Signaling

Figure 5

From: Divergent signaling pathways cooperatively regulate TGFβ induction of cysteine-rich protein 2 in vascular smooth muscle cells

Figure 5

ROCK mediates Smad-independent ATF2 activation by TGFβ. (A) VSMCs were pretreated with vehicle or ROCK inhibitor Y-27632 (10 μM) for 30 min before stimulation with TGFβ for 10 min. Western blot analyses were then performed to examine phosphorylation of ATF2 and Smad2. #P < 0.05 vs. TGFβ-stimulated vehicle group. (B) VSMCs were pretreated with vehicle or Y-27632 for 30 min before stimulation with or without TGFβ for 24 h. CRP2 expression was detected by Western blot analysis. #P < 0.05 vs. TGFβ-stimulated vehicle group. Representative blots of at least three independent experiments are shown.

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Cell Communication and Signaling

ISSN: 1478-811X