Figure 6
Differential effects of S100 proteins and TGFβ1 on mTOR signaling. BxPC3, Capan1, MiaPaCa2 and Panc1 remained unstimulated (NC, negative control) or were stimulated for 10 min with 50 mU insulin (positive control), 0.02 ng/ml TGFβ1 alone or combined with 50 nM NT-S100A8, 10 nM S100A8, 10 nM S100A9, 10 nM S100A8/A9 complex. Western blot showed the mTOR phosphorylation sites Ser2481 and Ser2448, the corresponding phosphorylation of S6 Ribosomal Protein at the Ser235/236 site (pS6RP) and β-actin, used as the control. Histograms show semi-quantification of band intensities after normalization against the negative control (O.D.; ImageJ software, v 1.47). Columns indicate mean values, bars indicate SD from two independ experiments.