Figure 5

Differential effects of S100 proteins and TGFβ1 on Akt signaling. BxPC3, Capan1, MiaPaCa2 and Panc1 remained unstimulated (NC, negative control) or were stimulated for 10 min with 50 mU insulin (positive control), 0.02 ng/ml TGFβ1 alone or combined with 50 nM NT-S100A8, 10 nM S100A8, 10 nM S100A9, 10 nM S100A8/A9 complex. Western blot show the Akt phosphorylation sites Thr³⁰⁸ and Ser⁴⁷³ and the corresponding non-phosphorylated Akt (panAkt), used as the control. Histograms show semi-quantification of band intensities after normalization against the negative control (O.D.; ImageJ software, v 1.47). Columns indicate mean values, bars indicate SD from two independ experiments.