Figure 8
PGE 2 -dependent effect on Wnt-target genes. Real-time PCR was used to determine the RQ value for Ctnnb1, Ptgs2, Ccnd1, and Mmp9. The expression of Ctnnb1 represented in fold change was 1, 0.97, 1.25, 1.55, 0.84, and 0.60, respectively. The fold change expression of Ptgs2 was 1, 0.56, 2.99, 4.59, 2.16, and 4.22. The fold change expression of Ccnd1 was 1, 3.68, 1.50, 1.99, 0.74, and 1.42. Mmp9 fold change expression was 1, 1.08, 2.19, 3.00, 2.16, and 2.68, respectively. The error bars represent + SEM and values were considered significantly different from control at *p < 0.05, **p < 0.01, and ***p < 0.001. Average measurements are from three independent experiments (N = 3).