Figure 2

PGE ₂ -dependent effect on final distance travelled from origin. (A) Final distance from origin was 65.6, 56.2, 21.3, 45.0 μm, respectively. The error bars represent + SEM and values were considered significant at *p < 0.05, **p < 0.01. (B) The Dispersion XY position plots illustrate the effect of PGE₂ on Wnt-induced behaviour, where addition of PGE₂ to Wnt-activated cells increased the final distance. Addition of H89 (PKA blocker) and Wort (PI-3K) blocker reduces the effect PGE₂. Measurements represent an average of 150 cells from three independent experiments (N = 3).