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Figure 4 | Cell Communication and Signaling

Figure 4

From: Downstream signaling and genome-wide regulatory effects of PTK7 pseudokinase and its proteolytic fragments in cancer cells

Figure 4

Protein phosphorylation profiling. A. Top panel, Protein phosphorylation profiling of p38a (T180/Y182), ERK1/2 (T202/Y204), Akt 1/2/3 (S473), CREB (S133), β-catenin protein, p53 (S15) and c-Jun (S63) in HT1080-scr, shPTK7, PTK7, sPTK7, cPTK7/622-1070, cPTK7/726-1070, Chz, shMT1-Chz and shMT1 cells. The spots were scanned and digitized. Pixel density of the spots is shown by the graphs in the bottom panel. B. A representative array of the HT1080 cell sample. The spots (boxed) of p38a (T180/Y182), ERK1/2 (T202/Y204), Akt 1/2/3 (S473), CREB (S133), β-catenin protein, p53 (S15) and c-Jun (S63) are numbered. C. Western blotting of phosphorylated and total cell c-Jun, CREB and ATF1 in HT1080, shPTK7, PTK7, sPTK7, cPTK7/622-1070, cPTK7/726-1070, Chz, shMT1-Chz and shMT1 cells.

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Cell Communication and Signaling

ISSN: 1478-811X