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Figure 4 | Cell Communication and Signaling

Figure 4

From: Intracellular signaling prevents effective blockade of oncogenic gp130 mutants by neutralizing antibodies

Figure 4

Effect on signaling by intracellular retention of de novo synthesized CAgp130. T-REx-293-WTgp130-YFP and T-REx-293-CAgp130-YFP were left untreated or expression was induced with 20 ng/ml dox for the indicated periods of time. Cells were simultaneously treated with 100 ng/ml brefeldin A or MeOH (vehicle). (A) Overall receptor expression was assessed by FACS analysis of the fluorescent tag (Additional file 1) and surface receptor expression was determined through staining with the gp130 Ab B-P8 and an APC labeled secondary Ab. Non-induced cells (filled histograms) were used as negative controls. (B) TCLs were analyzed by immunoblotting using Abs against pStat3(Y705), gp130 and actin as loading control.

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Cell Communication and Signaling

ISSN: 1478-811X