Figure 2

Pancreatic TCPTP deficiency decreases cerulein-induced pancreatic injury. A) Total pancreas lysates from wild type (WT) and panc-TCPTP KO (KO) mice were immunoblotted for TCPTP and Tubulin. B) Acute pancreatitis was induced by intraperitoneal injections of cerulein as detailed in Methods. Serum amylase and lipase were determined in control mice without (n = 9) and with (n = 12) cerulein and in panc-TCPTP KO mice without (n = 7) and with (n = 9) cerulein. C) TNFα, IL-6 and IL-1β expression (as assessed by quantitative real time PCR) in the pancreata of control mice without (n = 6) and with (n = 6) cerulein and panc-TCPTP KO mice without (n = 5) and with (n = 5) cerulein. D) Circulating levels of TNFα and IL-6 in serum of control mice without (n = 6) and with (n = 12) cerulein and panc-TCPTP KO mice without (n = 6) and with (n = 6) cerulein. (**; P ≤ 0.01) indicates significant difference between saline- and cerulein-injected mice, and (##; P ≤ 0.01) indicates significant difference between WT and KO mice.