Figure 4
From: Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels
Changes of IGF axis members in the liver and serum. Quantitative RT-PCR analyses of (A, B): Igf1 and (C, D): Igfbp1, Igfbp2 and Igfbp3 mRNA levels in hepatocytes isolated from 12-week-old male SAC-WT (white bars) (n = 7) and SAC-KO (black bars) (n = 7) (left diagrams) and female SAC-WT (white bars) (n = 7) and SAC-KO (black bars) (n = 5) (right diagrams) mice relative to that of β-actin. Values are presented as the means ± SEM; *, p < 0.05. (E, F): Serum analyses of circulating IGF-I and IGFBP-1 in male SAC-WT (white bars) (n = 9) and SAC-KO (black bars) (n = 8) mice and female SAC-WT (white bars) (n = 11) and SAC-KO (black bars) (n = 13) mice. Values are presented as the means ± SEM; *, p < 0.05.