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Figure 5 | Cell Communication and Signaling

Figure 5

From: Giα proteins exhibit functional differences in the activation of ERK1/2, Akt and mTORC1 by growth factors in normal and breast cancer cells

Figure 5

G i α proteins are required for phosphorylation of Shp2 but not FAK1 in response to EGF , bFGF and IGF - 1 . WT and Gi1/3 DKO MEFs were treated with (A) EGF (100 ng/ml), (B) bFGF (25 ng/ml), or (C) IGF-1 (20 ng/ml) for 0, 2, 5 or 15 min. p-FAK(Y397), p-Gab1(Y627), p-Shp2(Y508) and β-actin were detected by IB via anti-p-FAK(Y397), anti-p-Gab1(Y627), anti-p-Shp2(Y508) and anti-β-actin antibodies, respectively. Gab1 and Shp2 were also detected by IB (Additional file 5: Figure S5). Experiments were repeated two times and similar results were obtained.

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Cell Communication and Signaling

ISSN: 1478-811X