Figure 3

G _i α proteins regulate differential activation of ERK1 / 2 , Akt and mTORC1 in breast cancer cells in response to EGF , bFGF and IGF - 1 . MB231 cells were transfected with mock or siRNA duplexes (combination of G_i1α and G_i3α, or G_i1α, G_i2α and G_i3α). Cells were then treated with (A) EGF (100 ng/ml), (B) bFGF (25 ng/ml), or (C) IGF-1 (20 ng/ml) for the indicated time durations. p-AKT (S473), p-ERK (T202/Y204), p-S6 (S235/236), G_i1α (Gi1), G_i2α (Gi2), G_i3α (Gi3) or β-actin were detected by IB. The experiments were repeated two to three times and similar results were obtained.