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Figure 1 | Cell Communication and Signaling

Figure 1

From: Giα proteins exhibit functional differences in the activation of ERK1/2, Akt and mTORC1 by growth factors in normal and breast cancer cells

Figure 1

G i α proteins regulate differential activation of Akt, mTORC1 and ERK1 / 2 by growth factors. (A-F) WT and Gi1/3 DKO MEFs were treated with (A) EGF (100 ng/ml), (B) HB-EGF (100 ng/ml), (C) aFGF (25 ng/ml), (D) bFGF (25 ng/ml), (E) IGF-1 (20 ng/ml), and (F) insulin (1 μg/ml at the indicated time points. p-ERK (T202/Y204), p-AKT (S473), p-S6 (S235/236), or β-actin were detected by immunoblotting (IB) analysis. The experiments were performed more than six times and similar results were obtained. (G) WT and Gi1/3 DKO MEFs were treated with PDGF for 10 minutes (min) at the indicated doses. p-ERK(T202/Y204), p-AKT(S473), p-S6(S235/236), or β-actin were detected by IB. The experiment was repeated three times and similar results were obtained.

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Cell Communication and Signaling

ISSN: 1478-811X