Figure 4
From: NHERF2 is crucial in ERM phosphorylation in pulmonary endothelial cells
ERM cannot be phosphorylated in the absence of NHERF2. (A) Lysates of non transfected (ctr), non-siRNA or NHERF2 specific siRNA (sc-42522) treated cells were analyzed by Western blot using anti-EBP50, -NHERF2 and -actin antibodies. (B) Lysates of non transfected (ctr), non-siRNA or NHERF2 specific siRNA (sc-42522) treated cells without or with nocodazole (ND) challenge were analyzed by Western blot using antibodies against phospho-ERM, ERM, NHERF2 and actin as described in Methods. (C) NHERF2 or ROCK2 was immunoprecipitated from lysates of BPAEC. The IP complexes were probed for ROCK2 and NHERF2. (D) ROCK2 was immunoprecipitated from lysates of control, non-siRNA or NHERF2 specific siRNA treated cells. IP complexes were probed for ROCK2, ERM and NHERF2. Shown are representative data of at least 3 independent experiments.