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Figure 1 | Cell Communication and Signaling

Figure 1

From: Spatial and temporal aspects and the interplay of Grb14 and protein tyrosine phosphatase-1B on the insulin receptor phosphorylation

Figure 1

Effect of a phosphorylated BPS region of Grb14 on IR kinase and PTP1B activity. The domain organization of Grb14 and length of the BPS region is depicted (A). IR kinase activity was measured in the presence of either GST or GST-BPS-SH2 or mutant GST-BPS-SH2 (E373Q) in both non-phosphorylated and phosphorylated states (B), immunoblot of expressed proteins with anti-PY99 antibody and the same blot stripped and reprobed with anti-GST antibody (C). The fusion proteins (12 μM) that were used in the IR kinase activity (B) were further examined for their effect on PTP1B activity (D). Data are mean ± S.D., n = 3, **p < 0.001; *p < 0.05, P-Phosphorylated.

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Cell Communication and Signaling

ISSN: 1478-811X