Figure 3

Fz4-v1 function is required for dorsal fin development. (A-D) Embryos were injected at the 2- to 4-cell stage with 50 ng antisense Fz4/Fz4-v1 morpholino oligonucleotide (MO) alone (B, B’) (n = 10), or in combination with 500 pg fz4-v1 RNA (C, C’) (n = 29) or 500 pg fz4 RNA (D, D’) (n = 23). At the tailbud stage (st. 30) formation of the dorsal fin was compared to uninjected control embryos (A, A’) (n = 40). (A’-D’) Vibratome cross-sections of the embryos shown in A-D. White arrows indicate the dorsal fin. (E) Quantification of the experiment. (df) dorsal fin. (F) in situ hybridization for sox10 and twist mRNA in tailbud-stage embryos (st. 32) injected unilaterally with Fz4/Fz4-v1 MO or the combination of MO and fz4-v1 RNA. Asterices indicate the injected side of the embryos. (NI) non-injected side of the embryo, (wt) wild-type embryos. (G) Quantification of the CNC phenotypes.