Figure 3

Effect of acidity on tumor cells growth in hypoxic condition. DU145 (A-B) or PC3 (C-D) cells were labeled with CFDA-SE and then plated in single culture and in coculture with fibroblasts in a 1:2 ratio at 1% O₂ and at 20% O₂. The cells were grown at various pHe in DME supplemented with 25 mM hepes and 10 mM bicarbonate at 37°C and 5% CO₂. After 40 hours the cells were detached and analyzed by flow cytometry. The data show the proliferation index calculated on an average of six experiments. (*, p < 0.1; **, p < 0.05 and ***, p < 0.01).