Figure 1
From: The effects of CA IX catalysis products within tumor microenvironment
Effect of hypoxia and coculture on extracellular pH. Tumor and stromal cells were plated in single culture (2x105 for each cell type) and in coculture (105 DU145 or PC3 and 105 fibroblasts) at 37°C in unbuffered DME medium containing 1 mM bicarbonate in CO2 free atmosphere either at 1% O2 or 20% O2. Results show protons concentration values measured in DU145 single and coculture (A-B) and in PC3 single and coculture (C-D) after 24 hours calculated as a means ± SD from five independent experiments (*, p < 0.1; **, p < 0.05 and ****, p < 0.005).