Figure 3
From: Zinc inhibits osteoclast differentiation by suppression of Ca2+-Calcineurin-NFATc1 signaling pathway
Zinc regulates the mRNA levels of Nfatc1 and its target genes during BMM osteoclastogenesis. (A) BMMs were cultured in the present of M-CSF (30 ng/ml) and RANKL (120 ng/ml) for 4 days with or without ZnSO4 (100 μM). In RANKL-induced osteoclasts, mRNA expression of osteoclast marker genes (left panel) and fusion-related genes (right panel) were determined using real-time PCR. The results are expressed relative to each mRNA on day 4. (B) BMMs were cultured with M-CSF (30 ng/ml) and RANKL (120 ng/ml) in the presence or absence of ZnSO4 (100 μM) and FK506 (1 μM). After 24, 48, or 72 hours, total RNA was extracted from the cultured BMMs and mRNA levels were examined using real-time PCR. Data are presented as the mean ± S.D. of three independent experiments; * p < 0.05 compared to control and RANKL, respectively.