Figure 2

Zinc inhibits RANKL-induced osteoclast formation and fusion from BMMs. (A) BMMs were cultured for 4 days with M-CSF (30 ng/ml), RANKL (120 ng/ml), and various concentrations of ZnSO₄. The cells were stained for TRAP. (B) TRAP-positive multinucleated cells (nuclei ≥ 3) were counted using manual counting and a nuclei-counter plug-in for the Image J program. (C) TRAP activity was measured at 540 nm. Data are presented as the mean ± S.D. of three independent experiments; *P < 0.05 compared to control.