Figure 7
From: Sulindac activates NF-κB signaling in colon cancer cells
Sulindac sulfide induces transcriptionally-dependent up-regulation of A20 mRNA levels. qPCR analysis for A20 mRNA expression. HCT-15 cells were treated with 50 μM sulindac sulfide (SS), the vehicle DMSO (control line) or 10 ng/ml TNFα for the indicated time points. A20 gene expression was normalized to the house-keeping gene GAPDH. The data are presented as fold change ± SEM (from 4 to 7 independent experiments). (A) Cells were treated with the control, SS, TNFα or both in combination for the indicated time points. (B) Cells were pre-treated with 1 μM actinomycin D (ActD) and then treated with the indicated compounds for 4 hours.