Figure 4

Sulindac sulfide treatment induces up-regulation of NF-κB target genes in HCT116, SW480 and SW620 cells. (A) HCT116, SW480 and SW620 cells were treated with the indicated doses of sulindac sulfide (SS) or the control DMSO (equivalent to the highest dose SS) for 2 and 4 hours, after which lysates were collected and western blot analysis was performed for IκBα and the loading control β-actin. (B) Densitometry of IκBα levels normalized to β-actin. Error bars represent SEM (n = 3). * represents P < 0.05, + average from two data points. (C) qPCR analysis for A20, ICAM1 and IL-8 mRNA expression. Gene expression was normalized to the house-keeping gene GAPDH. The data are presented as fold change ± SEM (from 3 independent experiments).