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Figure 6 | Cell Communication and Signaling

Figure 6

From: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NFκB pathways which enhances transcription of MnSOD by accelerating IκBα degradation in normal and cancer cells

Figure 6

G2/M arrest along with reduction of H 2 O 2 level in the BTG2 expresser. HeLa cells were synchronized at G1/ S boundary by thymidine double block (TDB) before the following experiments. (A) Cell cycle analysis; Cells were harvested at the indicated times after TDB for FACS analyses. The X-axis corresponds to DNA content and Y-axis indicates number of cells. Note significant G2/M arrest in the BTG2 expresser at 8 h compared with the LacZ. (B) The cells in each phase were analyzed by ModFit software, and the statistical difference between the LacZ and the BTG2 expressers were evaluated by paired t-test. Note statistical significance in the G1 phase (p = 0.04) and the G2/M phase (p = 0.03) cells in the BTG2 expresser compared to LacZ control at 8 h. (C) Immunoblot analysis; Delayed synthesis of cyclin B1 in the BTG2 overexpresser at 4 h after TDB. (D) RT-PCR analysis showing the increased expressions of MnSOD and p21WAF1 in the BTG2 overexpresser at 6 h and at 8 h after TDB, respectively. (E) Induction of p21WAF1 expression in HeLa cells overexpressing BTG2 gene, proved by transfection and transduction of BTG2 gene. (F) Reduction of intracellular H2O2 in the BTG2 overexpresser than the LacZ control. HeLa cells were treated with 20 μM DCFDA for 15 min and analyzed by FACS. Note significant reduction of H2O2 level in the BTG2/TIS21 expresser than the LacZ control at 7 h after TDB.

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Cell Communication and Signaling

ISSN: 1478-811X